Mycobacterium tuberculosis variant bovis BCG strain:Pasteur 1721 Raw sequence reads. Mycobacterium tuberculosis variant bovis BCG strain:Pasteur 1721

The M. bovis BCG Pasteur strain 1721, used to create the sapM::Tn BCG mutant strain (Festjens et al, EMBO Mol Med 2011; Vandewalle et al, Nat Commun 2015), differs from the 1173P2 Pasteur vaccine strain by a K43R point mutation in the rpsL gene, conferring streptomycine resistance (Master et al, Cell Host Microbe 2008), which is useful to avoid contaminations during the lengthy and involved procedures in ordered transposon mutant library production. To investigate whether there are any additional variants between the WT BCG Pasteur and the sapM::Tn BCG mutant, we performed a whole-genome resequencing analysis on both strains (paired end 2 x 150 bp), mapping to the M. bovis BCG Pasteur 1173P reference genome, resulting in ± 80x average coverage of mapped reads.

本研究所用的牛分枝杆菌卡介苗（BCG）巴斯德株1721，是构建sapM::Tn BCG转座子插入突变株的亲本菌株（Festjens等, EMBO Mol Med 2011; Vandewalle等, Nat Commun 2015）。该菌株与1173P2巴斯德疫苗株的差异在于rpsL基因存在K43R点突变，该突变可赋予链霉素抗性（Master等, Cell Host Microbe 2008），此抗性标记可有效规避有序转座子突变体文库构建过程中冗长复杂的实验步骤所引发的污染问题。为探究野生型（WT）卡介苗巴斯德株与sapM::Tn BCG突变株之间是否存在额外遗传变异，我们对两株菌开展了全基因组重测序分析（双端2×150 bp测序，paired end），将测序读段（reads）比对至牛分枝杆菌BCG巴斯德株1173P参考基因组，最终获得的比对读段平均覆盖度约为80×。