Expression data from mouse heart after aortic binding surgery for 2 weeks

We found that tmbim1 is decreased in heart samples from patients with heart failure and in heart tissues from mice with aortic banding (AB)-induced cardiac hypertrophy. Furthermore, deleting Tmbim1 aggravates the AB-induced cardiac hypertrophy. To investigate the potential mechanism by which tmbim1 regulates cardiac hypertrophy, we treated wild type and tmbim1 knockout mice with aortic binding surgery for 2 weeks, and performed microarray to identify the expression pattern and the potential important targets. We used microarrays to detect the global gene expression in the hearts of wild type and Tmbim1 knockout mice after treatment with aortic binding surgery for 2 weeks and identified distinct classes of altered genes Eight weeks old wild type (n=3) and Tmbim1 knockout (n=3) mice were treated with aortic binding surgery for 2 weeks, and the hearts were dissected after. Total RNA was extracted and a total of 400ng RNA was used following Affymetrix instruction. 3 biological samples for each genotype

我们发现，心力衰竭患者的心脏样本以及主动脉缩窄（aortic banding, AB）诱导的心肌肥厚小鼠心脏组织中，Tmbim1的表达水平显著降低。进一步研究显示，敲除Tmbim1会加剧AB诱导的心肌肥厚。为探究Tmbim1调控心肌肥厚的潜在分子机制，我们以8周龄野生型（wild type）小鼠（n=3）与Tmbim1基因敲除小鼠（n=3）为实验对象，对其实施主动脉缩窄手术并持续处理2周，随后通过芯片（microarray）分析小鼠心脏的基因表达谱与潜在关键调控靶点。本次实验采用芯片技术检测上述经主动脉缩窄手术处理2周的小鼠心脏组织的全基因表达谱，筛选得到多类差异表达基因。实验过程中，提取小鼠心脏的总RNA，取总量400ng的RNA按照Affymetrix操作手册完成后续实验，每个基因型设置3个生物学重复样本。