Sanger sequencing of target and off-target genomic regions for gene-edited iPSC clones with SETBP1 genetic variants

This data set includes chromatograms generated using sanger sequencing of targeted regions of genomic DNA from clonal iPSC lines. The iPSC lines include clones generated using CRISPR/Cas9 homology directed repair to introduce genetic variants into SETBP1, and their wild-type controls. Additional files have been included in the data set to link chromatogram (ab1) files to specific iPSC clones for genomic regions across the variant in SETBP1 (SETBP1 clones genetic variant sanger sequencing.xslx) and top off-target sites (SETBP1 clones off-target sanger sequencing.xlsx).

本数据集包含来自克隆化诱导多能干细胞（induced pluripotent stem cell, iPSC）系的基因组DNA靶区域经桑格测序（Sanger Sequencing）生成的色谱图。该iPSC系涵盖通过CRISPR/Cas9同源定向修复（homology directed repair, HDR）技术向SETBP1基因引入遗传变异的克隆株，及其对应的野生型对照株。数据集还附带额外文件，用于将ab1格式的色谱图文件与特定iPSC克隆株进行关联：一类关联SETBP1基因变异位点所在及周边的基因组区域（对应文件：SETBP1 clones genetic variant sanger sequencing.xslx），另一类关联高优先级脱靶位点（对应文件：SETBP1 clones off-target sanger sequencing.xlsx）。