In vitro cultivation of "Prata Anã" banana Gorutuba clone in agitated and stationary liquid medium

ABSTRACT The aim of this study was to evaluate in vitro cultivation of banana Prata Anã clone Gorutuba in differents volumes of liquid medium in the stationary system and under agitation, comparing with the traditional cultivation in semisolid medium. The experiment was conducted in a completely randomized design with factorial 5 x 2 + 1, with five volumes (10, 15, 20, 25 and 30 mL) of liquid medium and two cropping systems (agitation and stationary) and control (semisolid medium), with 4 replicates, each plot had a bottle with 4 explants. The number of shoots/explant observed by the control on the third subculture differs from some treatments in liquid medium. In the fourth subculture, the number of shoots/explant of the control differed from all treatments in liquid medium, and cultivation under agitation system favored a number of shoots/explant significantly higher than that obtained by the stationary system. In the elongation and rooting phases, the results obtained in liquid medium, they were better than the control. Comparing the two systems of cultivation in liquid medium, we noted greater growth for plantlets which remained under agitation with the volume of 30ml. The cultivation in semisolid medium is more efficient in vitro multiplication phase, and in the phase of elongation and rooting the best system to be adopted is the cultivation in liquid medium under agitation to 30 mL.

摘要 本研究旨在评估普拉塔香蕉（Prata Anã）无性系Gorutuba在静止系统与振荡系统中，于不同体积液体培养基内的体外培养效果，并与传统半固体培养基培养方式开展对比。本实验采用完全随机析因设计5×2+1，设置5种液体培养基体积（10、15、20、25和30 mL）、2种培养系统（振荡培养与静止培养），并以半固体培养基作为对照组，每组设置4次重复，每个实验小区为1瓶搭载4个外植体的培养体系。第3次继代培养时，对照组的单外植体芽数与部分液体培养基处理组存在显著差异；第4次继代培养时，对照组单外植体芽数与所有液体培养基处理组均存在显著差异，且振荡培养系统的单外植体芽数显著高于静止培养系统。在伸长与生根阶段，液体培养基培养的效果均优于对照组。对比两种液体培养基培养系统，30 mL体积下的振荡培养组组培苗生长表现更佳。半固体培养基培养在体外增殖阶段效率更优，而在伸长与生根阶段，采用30 mL振荡液体培养基培养为最佳实施方案。