Supplementary Materials

<b>Supplementary Materials S1:</b> Translation-related candidate genes identified in this study.<b>Supplementary Materials S2:</b> Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of translation-related factors in RIRI-PA1 cells infected with AcMNPV-GFP at 24 hpi.<b>Supplementary Materials S3:</b> Predicted protein conformations of eIF4G and eIF6 from <i>S. frugiperda</i> and <i>P. americana</i>.<b>Supplementary Materials S4: </b>Expression profiles of AcMNPV genes and proteins in RIRI-PA1 cells infected with AcMNPV-GFP at 24 hpi, including FPKM values of viral genes determined by polysome-seq and LFQ intensity values of viral proteins determined by LC-MS/MS.<b>Supplementary Materials S5:</b> Protein–protein interaction models for <i>P. americana</i> and <i>S. frugiperda</i> eIF4G/eIF6 in complex with AcMNPV proteins.<b>Supplementary Materials S6:</b> Predicted interaction parameters for <i>P. americana</i> and <i>S. frugiperda</i> eIF4G/eIF6 with AcMNPV proteins (Tables S6-1 and S6-2).<b>Additional file:</b> Source data for figures (values underlying graphs and statistics).<b>Primer list:</b> Primer sequences used in this study.

<b>补充材料S1：</b>本研究鉴定获得的翻译相关候选基因。<b>补充材料S2：</b>感染携带绿色荧光蛋白的苜蓿银纹夜蛾核型多角体病毒（AcMNPV-GFP）24小时（24 hpi）后，RIRI-PA1细胞内翻译相关因子的基因本体论（Gene Ontology, GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析结果。<b>补充材料S3：</b>草地贪夜蛾（*S. frugiperda*）与美洲大蠊（*P. americana*）的真核翻译起始因子4G（eIF4G）及真核翻译起始因子6（eIF6）的预测蛋白质三维构象。<b>补充材料S4：</b>感染AcMNPV-GFP 24 hpi后RIRI-PA1细胞中苜蓿银纹夜蛾核型多角体病毒（AcMNPV）基因与蛋白的表达谱，涵盖通过多聚核糖体测序（polysome-seq）测得的病毒基因每百万片段每千碱基外显子片段数（FPKM）值，以及通过液相色谱-串联质谱（LC-MS/MS）测得的病毒蛋白无标记定量（LFQ）强度值。<b>补充材料S5：</b>美洲大蠊（*P. americana*）与草地贪夜蛾（*S. frugiperda*）的eIF4G/eIF6与苜蓿银纹夜蛾核型多角体病毒（AcMNPV）蛋白形成复合物的蛋白质相互作用模型。<b>补充材料S6：</b>美洲大蠊（*P. americana*）与草地贪夜蛾（*S. frugiperda*）的eIF4G/eIF6与AcMNPV蛋白之间的预测相互作用参数（含表S6-1与表S6-2）。<b>附加文件：</b>所有图表的源数据（包含绘图所用数值与统计分析数据）。<b>引物列表：</b>本研究中使用的所有引物序列。