DNA accessibility sites requiring SWI/SNF or β-catenin activity [ATAC-seq]

To reveal the effects of β-catenin knockout or acute SWI/SNF (BAF) inhibition on chromatin accessibility, we performed knockout of β-catenin or treated cells with 1 µM of BAF inhibitor BRM014 or DMSO vehicle control and performed ATAC-seq. Analysis of altered DNA accessibility in H295R cells revealed that sites with Steroidogenic Factor-1 (SF-1) motifs had the strongest loss of DNA accessibility. In HEK 293T cells, sites with motifs belonging to β-catenin binding partners (specifically YAP binding-partner TEAD, SOX, or FOXO motifs) had loss of DNA accessibility upon either BAF inhibition or β-catenin knockout. ATAC-seq in H295R or HEK 293T cells

为阐明β-连环蛋白（β-catenin）敲除或SWI/SNF（BAF）急性抑制对染色质可及性的影响，我们对β-连环蛋白进行敲除，或以1 µM浓度的BAF抑制剂BRM014及二甲基亚砜（DMSO）载体对照处理细胞，随后开展转座酶可及性染色质测序（ATAC-seq）实验。对H295R细胞中发生改变的DNA可及性进行分析后发现，携带类固醇生成因子1（Steroidogenic Factor-1，SF-1）基序的位点的DNA可及性丢失最为显著。在HEK 293T细胞中，无论经BAF抑制还是β-连环蛋白敲除处理，携带β-连环蛋白结合伴侣基序（具体为Yes相关蛋白（YAP）结合伴侣TEAD、SOX或FOXO基序）的位点均出现DNA可及性下降。本研究在H295R或HEK 293T细胞中开展了ATAC-seq