Tandem mass tag-based serum proteomic profiling revealed diabetic foot ulcer pathogenesis and potential therapeutic targets

Diabetic foot ulcer (DFU), one of the most serious complications of diabetes mellitus, is associated with a high amputation rate and decreased life quality. The impact of blood serum proteins on the occurrence and development of DFU has attracted a lot of interest. In this study, we aimed to define and compare the serum proteome of patients with DFU and healthy control (HC) to provide new insights into DFU pathogenesis. DFU patients and age- and sex-matched HCs were enrolled in this study (n = 54). We screened alterations in blood serum proteins from DFU patients and HC using a tandem mass tag (TMT) method based on liquid chromatography-mass spectrometry (LC-MS/MS) quantitative proteomics, and the differentially expressed proteins (DEPs) were further validated by parallel reaction monitoring (PRM) and enzyme-linked immunosorbent assay (ELISA). A total of 173 DEPs (100 up-regulated and 73 down-regulated) were identified between the DFU and HC groups (P

糖尿病足溃疡（Diabetic foot ulcer, DFU）是糖尿病最为严重的并发症之一，其伴随较高的截肢率与生活质量下降。血清蛋白对糖尿病足溃疡发生与发展的影响已受到广泛关注。本研究旨在明确并对比糖尿病足溃疡患者与健康对照（healthy control, HC）的血清蛋白质组，以期为糖尿病足溃疡的发病机制提供新见解。本研究共纳入54名受试者，包括糖尿病足溃疡患者及年龄、性别匹配的健康对照。本研究采用基于液相色谱-串联质谱（liquid chromatography-mass spectrometry, LC-MS/MS）定量蛋白质组学的串联质量标签（tandem mass tag, TMT）技术，筛选糖尿病足溃疡患者与健康对照的血清蛋白表达差异，并通过平行反应监测（parallel reaction monitoring, PRM）与酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）对差异表达蛋白（differentially expressed proteins, DEPs）进行验证。最终在糖尿病足溃疡组与健康对照组间共鉴定出173种差异表达蛋白（其中上调100种、下调73种，P