Long-term antibody response of CD46-IFNAR mice to immunization with MV-EDIII-ectoM.

aCD46-IFNAR mice were inoculated intraperitoneally (i.p.) twice with 104 TCID50 of MV-EDIII-ectoM at one month of interval. At 6 months, mice were boosted with 10 ��g of recombinant DV1 rEDIII-ectoM protein (from S2 cells) in Alugel adjuvant. At 9 months, mice were inoculated with 107 FFU of DV-1 FGA/NA d1d. bDetermined by ELISA on pooled heat-inactivated sera collected one month after immunizations or DV infection. cFRNT50 represents the highest serum dilution that reduced the number of DV focus-forming units (FFU) on Vero cells by at least 50% and dFRNT75 by at least 75%.

aCD46-IFNAR小鼠以1个月为间隔，经腹腔（i.p.）两次接种10^4 半数组织培养感染剂量（TCID50）的MV-EDIII-ectoM。于6月龄时，使用10 μg重组登革病毒1型（DV1）rEDIII-ectoM蛋白（源自S2细胞）辅以Alugel佐剂（Alugel adjuvant）对小鼠进行加强免疫。于9月龄时，小鼠接种10^7 蚀斑形成单位（FFU）的DV-1 FGA/NA d1d毒株。
b. 采用酶联免疫吸附试验（ELISA）对免疫或登革病毒（DV）感染后1个月收集的混合热灭活血清进行检测。
c. FRNT50指可使Vero细胞上的登革病毒蚀斑形成单位数量降低至少50%的最高血清稀释度；
d. FRNT75则指可使该指标降低至少75%的最高血清稀释度。