overview_calux_assays.xlsx

Two in vitro chemically activated luciferase gene expression (CALUX) reporter gene assays were applied to assess the activation of the estrogen and aryl hydrocarbon receptors (ERα and AhR), respectively. The two CALUX assays represent defined toxic modes of action and can serve as proxies for specific sources of pollution (van der Burg et al., 2013; De Baat et al., 2020). Estrogen receptor agonism (ERα CALUX), indicative of endocrine disruption, was used as a proxy for wastewater pollution (Välitalo et al., 2016) and aryl hydrocarbon receptor agonism (PAH CALUX), indicative of the induction of xenobiotic metabolism, as a proxy for industrial pollution (Neff et al., 2005). The ERα and PAH CALUX assays were performed by the BioDetection Systems laboratory (Amsterdam, The Netherlands) as described by De Baat et al. (2020). The effects of the extracts in the assays were expressed as bioanalytical equivalents (…EQ/L) of the reference compounds of the assays using the estimated sampled water volume of the passive samplers (Table 1).

本研究采用两种体外化学激活荧光素酶基因表达（CALUX）报告基因测定法，分别评估雌激素受体（ERα）和芳香烃受体（AhR）的激活情况。这两种CALUX测定法代表明确的毒性作用模式，可作为特定污染源的替代指标（van der Burg等，2013；De Baat等，2020）。其中，指示内分泌干扰的雌激素受体激动活性（ERα CALUX）被用作废水污染的替代指标（Välitalo等，2016）；指示外源代谢诱导的芳香烃受体激动活性（PAH CALUX）被用作工业污染的替代指标（Neff等，2005）。ERα和PAH CALUX测定法由荷兰阿姆斯特丹的BioDetection Systems实验室按照De Baat等（2020）所述方法进行。测定中提取物的效应通过被动采样器的估计采样水量，以测定参考化合物的生物分析当量（…EQ/L）表示（表1）。