Gene expression analysis of transplanted hiPSC-astrocyte progenitors generated from monozygotic twin pair discordant for schizophrenia into mouse brain

The humanized mice's frontal cortical tissues were used for transcriptomic analysis, as this brain area shows early involvement in pathophysiology of schizophrenia. We transplanted hiPSC-astrocyte progenitors from one monozygotic twin pair discordant for schizophrenia and one healthy control into neonatal mouse forebrains and analyzed mouse behavior at five and ten months, followed by gene expression profiling of the frontal cortices seven days later. Ribo-depleted RNA was sequenced with Illumina Next-seq 500 from transplanted mouse cortical samples

本研究以人源化小鼠的前额叶皮层组织为材料开展转录组分析，因该脑区在精神分裂症的病理生理进程中较早出现受累表现。我们将来自一对精神分裂症表型不一致的同卵双胞胎对（monozygotic twin pair discordant for schizophrenia）的人类诱导多能干细胞星形胶质细胞祖细胞（hiPSC-astrocyte progenitors），以及一名健康对照的同类细胞，移植至新生小鼠的前脑内；分别在小鼠达到5月龄和10月龄时开展行为学检测，于检测完成7天后采集前额叶皮层样本进行基因表达谱分析。本研究通过Illumina Next-seq 500测序平台，对移植小鼠皮层样本的核糖体RNA去除RNA（Ribo-depleted RNA）进行了测序。