Cardiac and pulmonary responses to diesel engine particulate in Apo E(-/-) mice. Mus musculus

Mice deficient in Apolipoprotein E (Apoe E(-/-)) vs wild type mice were exposed to diesel engine particulate by single dose intratracheal instillation. The transcript-level response of the hearts after 24h and of the lungs after 24 h and 5 days were subsequently analyzed by the 2-color microarray method. Keywords: stress response, disease state analysis Overall design: Four mice of the Apo E (-/-) strain were exposed to diesel engine particulate from the National Institute of Standards and Technology via intratracheal instillation. Controls consisted of 4 littermates exposed to saline vehicle. Four wild type mice were similarly exposed to diesel particulate and four to vehicle control. 24 hours after exposure, mice were humanely sacrificed by carbon dioxide asphyxiation and the hearts and lungs were collected and frozen for analysis of total RNA. 2 color microarrays were utilized to compare pooled RNA representing all four animals of each strain, and in each condition and in each tissue.

载脂蛋白E（Apolipoprotein E）缺陷型小鼠（Apoe E(-/-)）与野生型小鼠经单次气管内滴注暴露于柴油机颗粒物。随后，对暴露24小时后的心脏组织，以及暴露24小时、5天后的肺组织的转录水平应答，采用双色微阵列（2-color microarray）技术进行分析。
关键词：应激应答、疾病状态分析
总体设计：将4只Apo E(-/-)品系小鼠通过气管内滴注暴露于美国国家标准与技术研究院（National Institute of Standards and Technology, NIST）提供的柴油机颗粒物；对照组为4只同窝仔鼠，暴露于生理盐水载体。另有4只野生型小鼠以相同方式暴露于柴油机颗粒物，另外4只野生型小鼠则暴露于生理盐水作为载体对照。暴露24小时后，采用二氧化碳窒息法人道处死小鼠，采集心脏与肺组织并冻存，用于总RNA提取。本研究使用双色微阵列技术，对各品系、各暴露条件及各组织的4只小鼠的混合RNA进行对比分析。