SphK-produced S1P in somatic cells is indispensable for LH-EGFR signaling-induced mouse oocyte maturation

To further investigate the mechanism of SphK1/2 on oocyte maturation, we established Sphk1/2gc -/- and WT mice. We then performed transcriptomic analysis on the MGCs and cumulus cells of Sphk1/2gc-/- and WT mice at 2 h post-hCG. Comparative gene expression profiling analysis of RNA-seq data for the MGCs and cumulus cells of Sphk1/2gc-/- and WT mice at 2 h post-hCG.

为深入探究鞘氨醇激酶1/2（SphK1/2）调控卵母细胞成熟的作用机制，我们构建了生殖细胞特异性Sphk1/2敲除（Sphk1/2gc-/-）小鼠与野生型（Wild Type, WT）对照小鼠。随后，我们于人绒毛膜促性腺激素（human chorionic gonadotropin, hCG）处理后2小时，采集上述小鼠的壁层颗粒细胞（mural granulosa cells, MGCs）及卵丘细胞并开展转录组学分析。同时，针对hCG处理后2小时的Sphk1/2gc-/-与WT小鼠的MGCs及卵丘细胞的RNA测序（RNA-seq）数据，我们进行了比较基因表达谱分析。