Supplementary Information 2 Assay Methods & Results FINAL

The paper displays an exemplification of antiorganotrophism (tissue reduction) in vivo utilising ovine materials obtained from adult ewes. Ovine plasma was subjected to a bioassay-guided fractionation utilising a rat organometric assay that was conducted according to a standardised method over four days with intraperitoneal dosing. Organ shrinkage was readily provoked in what is a relatively crude assay in vivo. In cell culture experiments, shrinkage of rat bone marrow stem cells could be readily demonstrated using neat plasma from ovary-intact sheep, with such activity lacking in plasma from ovariectomised sheep

本文展示了采用成年母羊来源的绵羊源材料，在活体中验证抗器官营养效应（antiorganotrophism，即组织萎缩）的实例。研究人员以标准化方法构建为期四天的腹腔给药大鼠器官计量检测（rat organometric assay）模型，对绵羊血浆开展生物活性导向分级分离（bioassay-guided fractionation）。在该相对简易的检测体系中，器官萎缩可被轻松诱导。在细胞培养实验中，使用卵巢完整母羊的未稀释血浆即可成功证实大鼠骨髓间充质干细胞（rat bone marrow stem cells）发生萎缩，而去卵巢母羊的血浆则不具备该活性。