Leucobacter sp. TV5 Genome sequencing and assembly

Diverse bacteria under stress conditions produce polyhydroxyalkanoates (PHAs) as intracellular storage granules. The synthesis, accumulation and recovery of these biopolyesters have attracted high biotechnological interest in recent years (e.g., PHA-based bioplastics). However, some PHA producers can be facultative human pathogens, which can give rise to concern.Recently, we isolated PHA-producing bacterial strains from a sequencing batch reactor (SRB) for the production of PHAs at pilot-scale (ResUrbis Project; http://www.resurbis.eu). This study reports on the genome sequence analysis for the strain designed TV5, which was found to belong to Leucobacter of the family Microbacteriaceae (Actinobacteria). From the genomic data of Leucobacter sp. TV5 we detected genes involved in the production of PHAs, which may provide new insight to improve the ongoing research on PHA biosynthesis.The costs for the Next Generation Sequencing (NGS) were supported by INAIL-DIT (PAR 2016-2018).

处于胁迫条件下的多样化细菌会合成聚羟基烷酸酯（polyhydroxyalkanoates, PHAs）作为胞内储存颗粒。近年来，这类生物聚酯的合成、积累与回收工艺受到了生物技术领域的高度关注（例如基于PHAs的生物塑料）。不过，部分PHA产生菌属于兼性人类病原菌，这一点引发了安全顾虑。近期，我们从一个序批式反应器（sequencing batch reactor, SRB）中分离出了产PHA的细菌菌株，用于中试规模的PHA生产（ResUrbis项目；http://www.resurbis.eu）。本研究针对命名为TV5的菌株开展了基因组序列分析，经鉴定该菌株隶属于微杆菌科（Microbacteriaceae）的亮杆菌属（Leucobacter），属于放线菌门（Actinobacteria）。通过对Leucobacter sp. TV5的基因组数据分析，我们检测到了参与PHA合成的相关基因，这可为优化当前PHA生物合成相关研究提供新的见解。下一代测序（Next Generation Sequencing, NGS）的相关成本由INAIL-DIT（PAR 2016-2018项目）资助。