Is Increased Sinusoidal Flow in the Liver Remnant the primary Stimulus to Regeneration after partial Hepatectomy

The impact of portal arterialization on the liver parenchyma was studied in a series of six pigs with aortoportal shunting of liver segments II, III and IV (and 6 sham animals). Gene expression in the arterialized segments was compared to expression in sham animals. Primary endpoints were gene expression profiles in the hyperperfused segments at sampling time points 1, 5, 10, 30, 60, 90 minutes and 2, 3, 4 and 6 hours after shunt opening. Keywords: time course, treatment comparison Six pigs were treated with aortoportal shunting of segments II, III and IV and six animals were subject to sham surgery. Expression profiling was conducted by hybridizing each sample against a common reference, consisting of liver RNA from an unrelated animal..

本研究以6只接受第II、III、IV肝段主动脉门静脉分流术（aortoportal shunting）的猪与6只假手术组动物（sham animals）为实验对象，探究门静脉动脉化（portal arterialization）对肝实质（liver parenchyma）的影响。将动脉化肝段的基因表达水平与假手术组动物的对应基因表达水平进行对比分析。本研究的主要终点为分流开放后1、5、10、30、60、90分钟及2、3、4、6小时共10个采样时间点下，高灌注肝段（hyperperfused segments）的基因表达谱。
关键词：时间进程（time course）、治疗对照（treatment comparison）。本研究中，6头猪接受第II、III、IV肝段主动脉门静脉分流术，剩余6头动物仅接受假手术（sham surgery）。
基因表达谱分析通过将每份样本与由无关动物肝脏RNA制备的通用参照样本进行分子杂交完成。