The primary dipole of flipper probes

<strong>Table of contents</strong> <strong>1. Synthesis and characterization </strong> Compound <strong>8 </strong> Compound <strong>16</strong> Compound <strong>2</strong> Compound <strong>2’</strong> Compound <strong>10</strong> Compound <strong>18</strong> Compound <strong>3</strong> Compound <strong>3’</strong> <strong>2. Fluorescence spectroscopy</strong> Solvatochromism Solvatochromism labbook Absorbance Fluorescence Fluorescence quantum yields Labbook fluorescence quantum yields Fluorescein + <strong>2</strong>-<strong>3</strong>-<strong>2'</strong>-<strong>3'</strong> Quinine + <strong>3</strong>' hydrate Dynamic covalent ketone chemistry Kinetics of hydrate formation Water concentration dependence Fluorescence spectroscopy in LUVs Concentration dependence in LUVs Partition kinetics Partition coefficients <strong>3. CLSM and FLIM </strong> Confocal microscopy FLIM <strong>Data Format</strong>: Most data are in open format. Fluorescence data (OPJ) can be read in Origin Viewer (https://www.originlab.com/viewer/). CLSM file (lif) can be read in FIJI/ImageJ (https://fiji.sc/). FLIM data can be read in SymPhoTime software (Picoquant). <strong>Compound Numbers and Codes Correlations:</strong> <strong>1</strong> = JG-1-54 <strong>1’</strong> = JG-1-64 <strong>8 </strong>= JG-2-12 <strong>16 </strong>= JG-2-14 <strong>10</strong> = JG-2-5 = JG-2-18 <strong>18</strong> = JG-2-9 <strong>2 </strong>= JG-2-15 <strong>3 </strong>= JG-2-10 <strong>2’</strong> = JG-2-17 <strong>3’ </strong>= JG-2-16