Reprogramming fate of central memory CD8+T cells by targetig the transcriptional coregulator Tle3 [scRNA-seq]. Reprogramming fate of central memory CD8+T cells by targetig the transcriptional coregulator Tle3 [scRNA-seq]

The goal of this study is to determine if central memory (Tcm) and effector memory (Tem) CD8 T cells can be reprogrammed to change their fate. We demonstrate that genetic ablation of Tle3 can promote generation of Tcm cells at the expense of Tem cells, and this can occur during the effector phase of the immune response. Overall design: Single cell RNAseq analysis of WT or Tle3-deficient antigen-specific memory CD8 T cells isolated on day 30 after infection with LCMV-Armstrong.

本研究旨在探究中枢记忆（central memory, Tcm）与效应记忆（effector memory, Tem）CD8阳性T细胞是否可被重编程以改变其细胞命运。本研究证实，Tle3基因敲除可促进中枢记忆T细胞的生成，同时以效应记忆T细胞的产生为代价，且该过程可发生于免疫应答的效应阶段。实验总体设计为：对感染淋巴细胞脉络丛脑膜炎病毒-阿姆斯特丹株（LCMV-Armstrong）后第30天分离的野生型（WT）或Tle3缺陷型抗原特异性记忆CD8阳性T细胞开展单细胞RNA测序（Single cell RNAseq）分析。