Additional file 1: S1. of Catalogue of antibiotic resistome and host-tracking in drinking water deciphered by a large scale survey

Data filtration. S2. Illumina MiSeq sequencing for 16S rRNA genes. S3. Co-occurrence patterns among ARGs. Table S1. Basic information about drinking water samples. Table S2. Information about 16S rRNA gene analysis in drinking water samples. Table S3. The primers (including barcode, pad, and linker) used for 16S rRNA gene amplification. Table S4. Detected ARG types and corresponding abundances in drinking water. Table S5. Detected ARG subtypes and corresponding abundances in drinking water. Table S6. The resistance level in drinking water samples. Table S7. Abundance and frequency of ARG types in drinking water samples. Table S8. Co-occurring ARGs subtypes of network modules. Table S9. Basic information about 56 environmental samples for comparison of ARGs with drinking water samples. Table S10. Occurrence of 9 generalist ARG subtypes that were prevalent in drinking water. Table S11. Assembly statistics for drinking water metagenomic sequencing data. Table S12. Types and subtypes of ARGs carried by ACCs and their occurrence in drinking water. Table S13. Bacterial taxonomy of ARG-carrying contigs in the lowest annotation level and ARGs they carried. Table S14. Bacterial OTUs and their abundances in drinking water. Figure S1. Collection locations of drinking water samples. Figure S2. Cluster analysis of drinking water metagenomes based on abundance of ARG subtypes (Euclidean). Figure S3. Abundance of the top 50 ARG subtypes detected in tap water samples. Figure S4. Correlation-based network statistical analysis reveals co-occurrence patterns among ARG subtypes. Figure S5. Two-dimensional principal coordinate analysis plots show the ARG composition differences among the 81 environmental samples from 8 ecosystems. Figure S6. Two-dimensional principal coordinate analysis plots show the microbial community difference among tap water samples. (ZIP 3889 kb)

数据过滤。
补充材料2（S2）：针对16S rRNA基因的Illumina MiSeq测序。
补充材料3（S3）：抗生素抗性基因（Antibiotic Resistance Genes, ARGs）间的共现模式。
补充表S1：饮用水样本基础信息。
补充表S2：饮用水样本中16S rRNA基因分析相关信息。
补充表S3：用于16S rRNA基因扩增的引物（包含条形码（barcode）、间隔序列（pad）与接头（linker））。
补充表S4：饮用水样本中检出的ARG类型及其对应丰度。
补充表S5：饮用水样本中检出的ARG亚型及其对应丰度。
补充表S6：饮用水样本中的抗性水平。
补充表S7：饮用水样本中ARG类型的丰度与出现频率。
补充表S8：网络模块中共存的ARG亚型。
补充表S9：用于与饮用水样本进行ARG对比的56份环境样本基础信息。
补充表S10：9种在饮用水中普遍存在的泛宿主ARG亚型的检出情况。
补充表S11：饮用水宏基因组测序数据的组装统计信息。
补充表S12：ACCs所携带的ARG类型与亚型及其在饮用水中的出现情况。
补充表S13：最低注释层级下携带ARG的重叠群（contig）的细菌分类学信息及其所携带的ARG。
补充表S14：饮用水样本中的细菌操作分类单元（Operational Taxonomic Units, OTUs）及其丰度。
补充图S1：饮用水样本采集点位。
补充图S2：基于ARG亚型丰度（欧氏距离）的饮用水宏基因组聚类分析。
补充图S3：自来水样本中检出的前50种ARG亚型的丰度。
补充图S4：基于相关性的网络统计分析揭示ARG亚型间的共现模式。
补充图S5：二维主坐标分析（Principal Coordinate Analysis, PCoA）图谱展示8个生态系统的81份环境样本间的ARG组成差异。
补充图S6：二维主坐标分析图谱展示自来水样本间的微生物群落差异。（ZIP 3889 KB）