PMACS-86

Project title: PMACS-86Grant Information: AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarctionProject Description:· Animal Model: Myocardial Infarction· Animal Age: 8-12 Weeks· Animal Sex: Male· Animal Strain: GATA6Venus Reporter Mice· Number: 9 mice· Groups: 5 infarction, 4 sham surgery· Experimental Conditions: Mice were given MI via LAD ligation. Animals were sacrificed at day 3 post-infarction or sham surgery. Pericardial fluid was harvested. Pericardial fluid was incubated for hours in AMCF media stimulated with ng/mL LPS as well as golgiplug and golgistop to allow for intracellular staining . Results were analyzed via flow cytometry examining for Venus reporter expressionCode/Scripts: N/ALicenses: N/A

项目名称：PMACS-86 资助信息：美国心脏协会（AHA）资助项目编号9TPA34910007，研究主题为心包巨噬细胞在心肌梗死（Myocardial Infarction，MI）中的调控作用。
项目描述：
· 动物模型：心肌梗死模型
· 动物周龄：8~12周
· 动物性别：雄性
· 动物品系：GATA6 Venus报告基因小鼠（GATA6Venus Reporter Mice）
· 实验动物数量：9只
· 分组：5只心肌梗死造模组，4只假手术组
· 实验处理：通过左前降支（LAD）结扎构建心肌梗死模型，于造模或假手术后第3天处死小鼠，收集心包液。将心包液置于经ng/mL脂多糖（Lipopolysaccharide，LPS）刺激的AMCF培养基中孵育数小时，同时添加golgiplug与golgistop以实现细胞内染色。通过流式细胞术（flow cytometry）分析检测结果，以观察Venus报告基因的表达情况。
代码与脚本：N/A
许可协议：N/A