Identifying transcripts that are transcriptinoally regulated by CBFB and RUNX1 using RNAseq

Using RNAseq to identify differentially expressed transcripts between CBFB wild type (WT) and knockout (KO) or between RUNX1 wild type (WT) and knockout (KO) MCF10A cells. Overall design: Three repeats for CBFB KO and CBFB WT MCF10A cells. Four repeats for RUNX1 KO and RUNX1 WT MCF10A cells. One CBFB KO clone (#751) and two RUNX1 KO clones (5008 and 5010) were used.

本数据集通过RNA测序（RNAseq）技术，鉴定CBFB野生型（wild type, WT）与敲除（knockout, KO）、或RUNX1野生型（wild type, WT）与敲除（knockout, KO）的MCF10A细胞之间的差异表达转录本。整体实验设计：CBFB敲除组与CBFB野生型对照组MCF10A细胞均设置3次生物学重复；RUNX1敲除组与RUNX1野生型对照组MCF10A细胞均设置4次生物学重复。本次实验使用1株CBFB敲除克隆（#751）及2株RUNX1敲除克隆（5008与5010）。