Mechanomics AFM Set 02 - ECC4 vs TGBC

Entry is a part of <b>Mechanomics</b> collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:<i>Urbanska, M., Ge, Y.</i> et al.,<br><b><i>De novo identification of universal cell mechanics gene signatures</i></b>BiorXiv: https://doi.org/10.1101/2021.04.26.441418<i>;</i><i> </i><i>first published:</i><i> </i>27th April 2021<i>, last updated:</i><i> </i>4th Nov 2024<br>eLife: https://doi.org/10.7554/eLife.87930<i>; first published:</i><i> </i>23rd March 2023<i>, last updated:</i><i> </i>27th Dec 2024<br>This dataset contains mechanical measurement of ECC4 and TGBC18TKB (TGBC) carcinoma cell lines conducted with with atomic force microscope (AFM).Measurements were performed using Nanowizard 4 (JPK Instruments, Germany).Tip-less cantilevers (PNP-TR-TL, nominal spring constant k = 0.08 N m⁻¹, Nanoworld, Switzerland) decorated with a polystyrene bead of 5-µm diameter (PS-R-5.0, microParticles, Germany) each were used as the indenters.Following sub-folders are included under each sample:<br>• <b>Calibration </b>- contains data acquired during cantilever calibration<br>• <b>Ind </b>- indentation curves in .jpk-force format<br>• <b>Ind_processed-yyyy.mm.dd-hh.mm.ss</b> - results of processing of indentation curves with JPK data processing software (JPK DP, JPK Instruments)<br>• <b>Osc</b> - records of oscillatory measurements in .jpk-force format<br>• <b>Osc_processed-yyyy.mm.dd-hh.mm.ss</b> - results of processing of oscillatory measurements with JPK data processing software (JPK DP, JPK Instruments)<br>• <b>Pics</b> - bright-field images of cells acquired during the measurements, cantilever is located above the cell of interest

本条目属于<b>机械组学（Mechanomics）</b>数据集集合（DOI: 10.6084/m9.figshare.c.5399826），该集合包含支撑下述论文研究发现的相关材料：<i>Urbanska, M., Ge, Y.</i> 等人完成的<b><i>通用细胞力学基因特征的从头鉴定</i></b>。相关成果的预印本首次发布于BioRxiv：https://doi.org/10.1101/2021.04.26.441418，<i>首次发布时间：2021年4月27日，最后更新时间：2024年11月4日</i>；正式发表于eLife：https://doi.org/10.7554/eLife.87930，<i>首次发布时间：2023年3月23日，最后更新时间：2024年12月27日</i>。

本数据集包含采用原子力显微镜（atomic force microscope, AFM）对ECC4与TGBC18TKB（简称TGBC）癌细胞系开展的力学性能测试数据。测试使用德国JPK Instruments公司的Nanowizard 4型原子力显微镜完成。实验所用压头为经5μm直径聚苯乙烯微球（PS-R-5.0，德国microParticles公司）修饰的无针尖悬臂梁（PNP-TR-TL，标称弹簧常数k=0.08 N·m⁻¹，瑞士Nanoworld公司）。

每个样本下包含如下子文件夹：
• <b>Calibration</b>：包含悬臂梁校准过程中采集的原始数据
• <b>Ind</b>：格式为.jpk-force的压痕曲线数据
• <b>Ind_processed-yyyy.mm.dd-hh.mm.ss</b>：采用JPK数据处理软件（JPK DP, JPK Instruments）对压痕曲线进行处理后得到的分析结果
• <b>Osc</b>：格式为.jpk-force的振荡测量记录数据
• <b>Osc_processed-yyyy.mm.dd-hh.mm.ss</b>：采用JPK数据处理软件（JPK DP, JPK Instruments）对振荡测量数据进行处理后得到的分析结果
• <b>Pics</b>：测试过程中采集的细胞明场成像图，图中悬臂梁位于目标细胞上方