Effect of overexpression of ESRP1 on gene expression of SGC7901 gastric cancer cells

RNA-binding proteins and their mediated alternative splicing play important roles in tumor cell invasion and migration. Here, we report that ESRP1 is a key regulator of gastric cancer cell metastasis. Overexpression of ESRP1 inhibits the invasion and migration of gastric cancer cells, in vivo and in vitro. Furthermore, we found that ESRP1 causes a wide range of alternative splicing events, and ESRP1-mediated CLSTN1 exon skipping may be a key mechanism for its inhibition of gastric cancer cell invasion and metastasis. Taken together, our data provide a molecular framework for the role of ESRP1 in gastric cancer development. Overall design: In order to study the role and mechanism of ESRP1 in the invasion and migration of gastric cancer cells, we established the SGC7901 gastric cancer cell line with stable overexpression of ESRP1. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells.

RNA结合蛋白（RNA-binding proteins）及其介导的可变剪接（alternative splicing）在肿瘤细胞侵袭与迁移中发挥重要调控作用。本研究发现，ESRP1是胃癌细胞转移的关键调控因子。体内外实验均证实，ESRP1过表达可抑制胃癌细胞的侵袭与迁移能力。进一步研究显示，ESRP1可诱导广泛的可变剪接事件，且其介导的CLSTN1外显子跳跃可能是其抑制胃癌细胞侵袭与转移的核心分子机制。综上，本研究数据为ESRP1在胃癌发生发展中的作用提供了分子调控框架。整体实验设计：为探究ESRP1在胃癌细胞侵袭与迁移中的作用及分子机制，我们构建了稳定过表达ESRP1的SGC7901胃癌细胞系。随后，基于两种不同细胞的RNA测序（RNA-seq）数据开展基因表达谱分析。