Additional file 1: of The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis

Figure S1. Schematic diagram of MdMYB30 genomic and cDNA sequences. UTR: untranslated region. Figure S2. Changes in wax load, composition, and cuticular wax crystal morphology of WT plants and MdMYB30 ectopic expression plants detected by scanning electron microscopy. a Total cuticular wax content of leaves, calculated per unit area of 6-week-old Arabidopsis from the WT and MdMYB30 ectopic expression lines. b Cuticular wax composition of alkanes, alcohols, aldehydes, fatty acids, ketones, and esters on the stem surfaces of WT and MdMYB30 ectopic expression plants analyzed by GC-MS. Wax constituents are grouped by carbon chain length and chemical class. c Wax crystal morphology of 6-week-old Arabidopsis leaves from the WT and MdMYB30 ectopic expression lines (scale bars: 10 μm). Error bars represent standard deviation (SD; n = 3). Data are mean ± SD of three independent replicates. Different lowercase letters indicate significant differences at P < 0.05. Figure S3. Diagram representing the genomic structure and primer sets (indicated by P1-P3) analyzed in the MdKCS1 genes by ChIP-qPCR. White boxes represent primer sets, and black boxes represent ATG starting open reading frame (ORF). Table S1. Total epicuticular wax on wild type Arabidopsis, MdMYB30 OE4, OE5, and OE6 surface areas. Table S2. Epicuticular wax component (μg/dm2) in stems of wild-type Arabidopsis, MdMYB30 OE4, OE5, and OE6. Table S3. Primers used in this study. File S1. The amino acid sequences of MdMYB30 and homologs from 12 other plant species to analyze the phylogenetic relationships. (ZIP 1525 kb)

图S1　MdMYB30基因组与cDNA序列示意图。UTR：非翻译区（untranslated region）。
图S2　通过扫描电子显微镜检测的野生型（Wild Type，WT）植株与MdMYB30异位表达植株的蜡质负载量、组成及表皮蜡质晶体形态变化。a 6周龄野生型拟南芥及MdMYB30异位表达株系叶片单位面积的总表皮蜡质含量。b 通过气相色谱-质谱联用（GC-MS）分析野生型与MdMYB30异位表达植株茎表面的烷烃、醇类、醛类、脂肪酸、酮类及酯类表皮蜡质组成；蜡质组分按碳链长度与化学类别进行分组。c 6周龄野生型及MdMYB30异位表达株系拟南芥叶片的蜡质晶体形态（标尺：10 μm）。误差棒代表标准偏差（SD；n=3）。数据为3次独立重复实验的平均值±标准偏差。不同小写字母表示在P<0.05水平下存在显著差异。
图S3　本研究中通过染色质免疫共沉淀-定量聚合酶链式反应（ChIP-qPCR）分析MdKCS1基因的基因组结构与引物对（标注为P1-P3）示意图。白色方框代表引物对，黑色方框代表ATG起始的开放阅读框（open reading frame，ORF）。
表S1　野生型拟南芥、MdMYB30过表达株系OE4、OE5及OE6的单位面积表皮总蜡质含量。
表S2　野生型拟南芥、MdMYB30过表达株系OE4、OE5及OE6茎中的表皮蜡质组分含量（μg/dm²）。
表S3　本研究使用的引物序列。
补充文件S1　用于分析系统发育关系的MdMYB30及其他12种植物的同源蛋白氨基酸序列（ZIP 1525 kb）