Cooperative ETS Transcription Factors Enforce Adult Endothelial Cell Fate and Cardiovascular Homeostasis [Liver_InVivo_ATACseq]. Cooperative ETS Transcription Factors Enforce Adult Endothelial Cell Fate and Cardiovascular Homeostasis [Liver_InVivo_ATACseq]

Bulk ATACseq of mouse in vivo liver vascular Endothelial samples either control or Erg and Fli1 knock out. Overall design: Mice were intravitally injected with VE-cadherin antibody. Tissues were dissociated by Collagenase Dispase digestion and stained for CD45 and CD31. Endothelial cells were sorted as live (Dapi negative), CD45 negative, CD31 positive, VE-cadherin positive cells. Sorted endothelial cells were processed for ATAC-seq using the Zymo DNA Clean & Concentrator-5 kit and using NEBNext X2 MasterMix (New England Biolabs).

小鼠体内肝脏血管内皮样本的批量转座酶可及性染色质测序（ATAC-seq）数据集，分为对照组与Erg、Fli1基因敲除组。实验设计：对小鼠经玻璃体内注射VE-钙粘蛋白（VE-cadherin）抗体；采用胶原酶Dispase（Collagenase Dispase）消化法解离组织，随后对细胞进行CD45与CD31染色；通过流式分选获取活细胞（DAPI阴性）、CD45阴性、CD31阳性且VE-钙粘蛋白阳性的内皮细胞；分选得到的内皮细胞使用Zymo DNA Clean & Concentrator-5试剂盒及NEBNext X2 MasterMix（纽英伦生物技术有限公司，New England Biolabs）完成文库构建与测序。