Metagenome analysis of bacterial endophytes in corm and shoot tissues of Crocus sativus L.. Crocus sativus L.

This study was carried out to assess the extent of bacterial diversity in corm and shoot tissues of Crocus sativus (saffron). The project was initiated based on the background that only a small fraction of endophytic bacteria are culturable by routine isolation techniques used in laboratory. It is our interest to explore the unculturable endophytic bacterial diversity of Crocus sativus, from which we isolated culturable bacterial endophytes so that we would explore more comprehensive bacterial endophytic microbiome of Crocus sativus. Total DNA was extracted from corm and shoot tissues homogenate of the plant. The DNA samples from corm and shoot (designated as E1 and E2) were used for microbiome data analysis through V3 region profiling of 16S rRNA gene.

本研究旨在评估藏红花（Crocus sativus）球茎与茎组织中的细菌多样性水平。本项目的启动背景为：实验室常规分离技术仅能培养出极小部分内生细菌（endophytic bacteria）。我们旨在探究藏红花的未培养内生细菌多样性，并在此基础上分离可培养的内生细菌，以全面解析藏红花的内生细菌微生物组（microbiome）。研究人员从该植物的球茎和茎组织匀浆中提取总DNA，将球茎、茎组织来源的DNA样本分别命名为E1和E2，随后通过对16S rRNA基因的V3可变区进行扩增子谱分析，开展微生物组数据分析。