GeneChip Expression Array

We transfected cDNA of human umbilical vein endothelial cells into rat myeloma cells, and sorted cells bound to patient IgG. Sorted cells were analyzed using the Affymetrix Human Genome U133 Plus 2.0 Array to investigate the cDNA inserted into the sorted cells. Array data was processed by Affymetrix GeneChip Command Console Software and Affymetrix Expression Console Software. No techinical replicates were performed.我们将人脐静脉内皮细胞（human umbilical vein endothelial cells）的互补脱氧核糖核酸（cDNA）转染至大鼠骨髓瘤细胞内，随后筛选结合患者免疫球蛋白G（IgG）的细胞。采用Affymetrix人类基因组U133 Plus 2.0基因芯片对分选得到的细胞进行分析，以此探究插入至分选细胞中的cDNA序列。芯片数据通过Affymetrix GeneChip Command Console软件及Affymetrix Expression Console软件完成处理。本实验未设置技术重复。