遇见数据集

Table S2. Detailed information on the offspring from the analysed crosses. from Interaction between sex-determining genes from two species: clues from <i>Xenopus</i> hybrids

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Detailed information on the offspring from the analysed crosses (X44, X54, X67, X68, X82, and X197). Cross X67 was designated to produce allopentaploid hybrids. DoD indicates the number of days of development of the tadpole when analysed. The phenotype was preliminary declared according to the morphological appearance of the gonads under a stereoscope. <i>X. tropicalis</i> and <i>X. laevis</i> sex chromosomes (Sex Chr.) were genotyped as stated in the material and methods section. Sample IDs labelled in grey indicate the analysed animals that were found dead although in relatively good condition. Colour labelled individuals were not included in the total count due to: the absence of gonads in the sample (X44.17, yellow); not genotyped due to degraded DNA (X67.8, blue), and discordant results explained by a <i>dm-w</i> false negative or a recombinant W chromosome with a Z-linked marker (X54.4, orange). The Z chromosome from <i>X. tropicalis</i> in red indicates the possibility that this chromosome is, in fact, a recombinant W chromosome with a Z-allele for the marker used (after recombination between the sex-determining allele (W) and the Z-linked marker in that family). GC=Germ cells; PM=Post-metamorphic; na=not analysed; undif.=undifferentiated.

本数据集包含本次分析的6组杂交组合(X44、X54、X67、X68、X82及X197)子代的详细信息。其中杂交组合X67的设计目标为获得异源五倍体杂种。DoD代表分析时受试蝌蚪的发育天数。表型初步依据体视镜下性腺的形态外观进行判定。热带爪蟾(*X. tropicalis*)与非洲爪蟾(*X. laevis*)的性染色体(Sex Chr.)已按照材料与方法部分所述完成基因分型。以灰色标注的样本ID对应虽状态相对良好但已死亡的受试个体。带有颜色标注的个体未被计入总计数,具体原因如下:样本中未检出性腺(如X44.17,黄色标注);因DNA降解未完成基因分型(如X67.8,蓝色标注);检测结果不一致,此类情况可归因于dm-w假阴性,或是携带Z连锁标记的重组W染色体(如X54.4,橙色标注)。红色标注的热带爪蟾Z染色体,提示该染色体实际可能为携带所用标记Z等位基因的重组W染色体(源于该家系中性别决定等位基因(W)与Z连锁标记之间发生重组)。GC=生殖细胞(Germ cells);PM=变态后(Post-metamorphic);na=未分析(not analysed);undif.=未分化(undifferentiated)。

提供机构:
The Royal Society
创建时间:
2021-06-09
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