Whole-organism C. elegans L2 GRH-1 ChIP-seq (experiment 2 of 2). Whole-organism C. elegans L2 GRH-1 ChIP-seq (experiment 2 of 2)

We performed ChIP-seq to determine chromatin regions binding Transcription Factor GRH-1, and thereby potential binding motifs and sites, in C. elegans (larval stage 2) whole organisms. Overall design: One sample was used as "input", ie non-specific pulldown. 1 new GRH-1-specific pulldown sample was generated. Samples were collected at two time points to broadly cover the L2 stage, before pooling. Additionally, two samples (1 input and 1 pulldown, 500mM salt) from experiment 1 were re-extracted, prepared and sequenced.

本研究通过染色质免疫共沉淀测序（ChIP-seq），旨在鉴定秀丽隐杆线虫（C. elegans）第二幼虫期（L2期）整体个体中结合转录因子GRH-1的染色质区域，进而推导其潜在结合基序与结合位点。实验设计概况：设置1份“Input对照”样本，即非特异性下拉样品；同时构建1份全新的GRH-1特异性下拉样本。为覆盖第二幼虫期的宽泛时间范围，我们在样本混合前于两个时间点收集样品。此外，将首轮实验中获得的2份样本（1份Input对照与1份500mM盐洗脱下拉样本）进行二次提取、文库制备并测序。