Does mechanical damage on soybean induces the production of flavonoids?

Abstract The response of plants to grazing includes the production of chemical defense compounds such as proteases inhibitors and secondary metabolites as flavonoids, which makes them less palatable to feeding and negatively affecting the physiology of insects. The aim of this study was to evaluate the phytochemical response of soybean cultivars (Glycine max (L.) Merrill) resistant (IAC-17, IAC-24) and susceptible (IAC-P1) to insects after mechanical damage. These cultivars were mechanically injured, and after 24 hours samples of these plants were analyzed by HPLC to identify and quantify flavonoids. The flavonoids daidzein, quercetin, and rutin were quantified, with the highest concentration of daidzin in soybean cultivars after mechanical damage. Rutin was biosynthesized by IAC-24. The cultivars IAC-PL1, IAC-17, and IAC-24 did not show a flavonoid response to mechanical damage. The soybean cultivars are not dependent on mechanical damage to produce flavonoids.

摘要 植物对放牧的响应包括合成蛋白酶抑制剂（protease inhibitors）、黄酮类（flavonoids）等次生代谢物在内的化学防御化合物，此举可降低植物适口性，并对昆虫生理状态产生负面影响。本研究旨在评估经机械损伤后，抗虫大豆品种（Glycine max (L.) Merrill，IAC-17、IAC-24）与感虫大豆品种（IAC-P1）的植物化学响应。研究人员对上述品种施加机械损伤处理，并在24小时后采集植株样本，通过高效液相色谱（HPLC）对黄酮类物质进行定性与定量分析。本研究对染料木素（daidzein）、槲皮素（quercetin）与芦丁（rutin）三种黄酮类物质开展定量检测，结果显示机械损伤后的大豆品种中大豆苷（daidzin）浓度最高；芦丁（rutin）由IAC-24品种生物合成。IAC-PL1、IAC-17与IAC-24三个品种未在机械损伤后表现出黄酮类物质响应。供试大豆品种合成黄酮类物质并不依赖于机械损伤。