Dataset: “High-throughput chemical toxicity testing for beneficial insects: The value of coated vials”

This repository contains all the data and scripts required to replicate the analyses presented in Thia et al. (2025) “High-throughput chemical toxicity testing for beneficial insects: The value of coated vials”, published in <i>Journal of Economic Entomology </i>(https://doi.org/10.1093/jee/toaf155).This study contains chemical toxicity data for larval and adult life stages of the ladybeetle, <i>Hippodamia variegata</i>, and the adult life stage of the parasitoid wasp, <i>Diaeretiella rapae</i>. There are 3 experiments comprising this dataset. Experiment 1 involved the use of coated vial to identify dose-response ranges for these natural enemies to three pesticides (alpha-cypermethrin, dimethoate, and sulfoxaflor). Experiment 2 then utilised these dose-response ranges to compare mortality in coated vial versus Potter tower spray dish assays at predicted LC20-, LC50-, and LC80-values for each pesticide. Experiment 3 was a validation experiment testing the interaction between pesticide application and vessel: coated vials, coated dishes, and Potter tower sprayed dishes.All data analyses are run in R. You can use the R project file, <i>NE_toxicity_testing.Rproj</i> to manage the analyses. Simply open this R project file in the main directory and make sure the <i>Data/</i>, <i>Functions/</i> and <i>Results/</i> directory are all in the same place. There is a separate script for each natural enemy: <i>1_Ladybird_larvae_analysis.R</i>, <i>2_Ladybird_adults_analysis.R</i>, and <i>3_Parasitoid_analysis.R</i>. A final script, <i>4_Combined_plots.R</i>, is used to create composite plots for all natural enemies.

本仓库包含复现Thia等人（2025）发表于《经济昆虫学期刊》（*Journal of Economic Entomology*，DOI: 10.1093/jee/toaf155）的论文《高通量有益昆虫化学毒性测试：包被试管的应用价值》中所述分析所需的全部数据与脚本。本数据集涵盖异色瓢虫（*Hippodamia variegata*）幼虫与成虫两个虫态，以及菜蚜茧蜂（*Diaeretiella rapae*）成虫的化学毒性数据。本数据集包含3组实验：实验1采用包被试管法，明确上述两类天敌昆虫对3种农药（高效氯氰菊酯、乐果、氟啶虫胺腈）的剂量-反应范围；实验2依托前述剂量-反应范围，在各农药的预测LC20、LC50与LC80值条件下，对比包被试管法与Potter喷雾塔喷雾皿法的昆虫死亡率；实验3为验证实验，测试农药施用方式与容器类型的交互效应，涉及的容器包括包被试管、包被皿以及Potter喷雾塔喷涂皿。所有数据分析均基于R语言完成，可通过项目文件<i>NE_toxicity_testing.Rproj</i>管理分析流程：只需在主目录中打开该R项目文件，并确保<i>Data/</i>、<i>Functions/</i>与<i>Results/</i>三个目录处于同一层级即可。针对每一类天敌昆虫均配有独立分析脚本：<i>1_Ladybird_larvae_analysis.R</i>、<i>2_Ladybird_adults_analysis.R</i>及<i>3_Parasitoid_analysis.R</i>；最后一份脚本<i>4_Combined_plots.R</i>用于生成覆盖所有天敌昆虫的组合绘图。