Microassay analysis of the lncRNAs expression profile in human aortic vascular smooth muscle cells(hAoSMCs) treated with PDGF-BB at 24 h.. Microassay analysis of the lncRNAs expression profile in human aortic vascular smooth muscle cells(hAoSMCs) treated with PDGF-BB at 24 h.

Atherosclerosis is the most important mechanism of cardiovascular and cerebrovascular diseases and has become one of the most serious diseases that threaten human health. Vascular smooth muscle cells (VSMCs) are the main cellular components that constitute the structure of the blood vessel wall. The excessive proliferation and migration are pivotal pathological basis of atherosclerosis, in-stent restenosis, pulmonary hypertension and other vascular proliferative diseases. PDGF-BB is a potent proliferative agent for VSMCs, but the specific mechanism of PDGF-BB signaling pathway involved in biological processes such as proliferation and migration of VSMCs is not elusive. Overall design: Used the lncRNAs microarray technology to screen the target lncRNA in hAoSMCs proliferation model treated with PDGF-BB (20ng/mL).Total RNAs of 3 replicates of hAoSMCs treated with vehicle or PDGF-BB at 24 h were sequenced and analyzed for identification of novel lncRNAs.

动脉粥样硬化（Atherosclerosis）是心脑血管疾病最重要的发病机制，现已成为威胁人类健康的最严重疾病之一。血管平滑肌细胞（Vascular smooth muscle cells, VSMCs）是构成血管壁结构的主要细胞组分。其过度增殖与迁移是动脉粥样硬化、支架内再狭窄、肺动脉高压等血管增殖性疾病的关键病理基础。血小板衍生生长因子-BB（PDGF-BB）是诱导VSMCs增殖的强效因子，但PDGF-BB信号通路参与VSMCs增殖、迁移等生物学过程的具体机制并非难以捉摸。总体实验设计：采用lncRNAs微阵列技术，在经20ng/mL PDGF-BB处理的人主动脉平滑肌细胞（human aortic smooth muscle cells, hAoSMCs）增殖模型中筛选目标lncRNA。收集经溶剂对照或PDGF-BB处理24h的hAoSMCs的3个生物学重复样本的总RNA，进行测序及分析以鉴定新型lncRNA。