TwinNetworkDataset032

The data comprises bright-field time-lapse images of zebrafish embryos acquired in multiple batches within multi-well plates using an Acquifer Imaging Machine. Embryos from incrosses of Tg(sebox:EGFP) and incrosses of TE zebrafish strains were used for imaging. Small-molecule signaling pathway modulator LDN-193189 was added to well A2 with a final concentration of 3.5 µM and to well A3 with a final concentration of 4 µM. Small-molecule signaling pathway modulator SB-505124 was added to wells B1-B3 with final concentrations of 4 µM in well B1, 5 µM in well B2, and 6 µM in well B3. Well A1 did not receive small-molecule signaling pathway modulator treatment. Imaging started with embryos between 2-3 hpf and ended with embryos between 27-28 hpf. Images of embryos were acquired at 180 acquisition time points with a time interval of 501 s between each acquisition time point. Individual embryo segments were identified and extracted using a trained neural network for object detection. Within this experiment folder, data are organized by microscope position and embryo number.

该数据集包含采用Acquifer成像仪（Acquifer Imaging Machine）在多孔板中完成多批次采集的斑马鱼胚胎明场延时序列图像。成像所用的胚胎来源于Tg(sebox:EGFP)品系的互交子代以及TE品系斑马鱼的互交子代。将小分子信号通路调节剂LDN-193189加入孔板A2孔，终浓度为3.5 μM；加入A3孔，终浓度为4 μM。将小分子信号通路调节剂SB-505124加入B1至B3孔，其中B1孔终浓度为4 μM，B2孔为5 μM，B3孔为6 μM。A1孔未添加任何小分子信号通路调节剂进行处理。成像始于受精后2-3 hpf（hours post fertilization，受精后小时数）的胚胎，终止于受精后27-28 hpf的胚胎。共在180个采集时间点采集胚胎图像，各采集时间点之间的时间间隔为501秒。采用经过训练的目标检测神经网络，对单个胚胎片段进行识别与提取。本实验文件夹内的数据按照显微镜成像位置与胚胎编号进行组织。