Molecular characterisation of ribonucleic acid (RNA) interference in Fusarium circinatum

Identification and inference of the evolutionary relationship of seven known Ribonucleic acid (RNA) interference components Fusarium circinatum, its closely related species as well as other fungi outside of the genus. Twenty-one Fusarium species and thirteen species from other genera were chosen. The genes of interest include Argonaute, Dicer, RNA-dependent RNA polymerase, Replication protein A1 and A2, Mitochondrial ribosomal protein L3 (MRPL3) and Quelling Deficient-2 interating protein (QIP). A proof of concept of the functionality of RNA interference (RNAi) in Fusarium circinatum. These was done by applying double-stranded RNA targeting a fluorescence gene in Fusarium circinatum.

本研究旨在鉴定并推断七种已知的核糖核酸（RNA）干扰组分的进化关系，研究对象涵盖环镰孢（Fusarium circinatum）、其近缘物种以及该属之外的其他真菌。本数据集共选取21种镰孢属物种与13种其他属的真菌。所关注的靶标基因包括阿戈纳特（Argonaute）蛋白、Dicer酶、RNA依赖的RNA聚合酶、复制蛋白A1与A2、线粒体核糖体蛋白L3（MRPL3）以及沉默缺陷2互作蛋白（QIP）。此外，本研究还验证了环镰孢中RNA干扰（RNAi）的功能可行性，具体通过向环镰孢中施加靶向荧光基因的双链RNA（double-stranded RNA）完成该验证。