Human colon epithelial cells treated with Clostridium difficile Toxins A and B. Homo sapiens

Toxin A and B from Clostridium difficile are the primary virulence factors in Clostridium difficile disease. The changes in gene transcription of human colon epithelial cells were investigated in vitro in order to better understand the many effects of both toxins. Overall design: HCT-8 cells were treated with 100 ng/ml of either Toxin A or B (TcdA or TcdB). RNA was isolated 2, 6, and 24 hours after addition of toxin from untreated and toxin-treated cells.

艰难梭菌（Clostridium difficile）分泌的毒素A与毒素B是艰难梭菌感染疾病的主要毒力因子。为更清晰地阐明这两种毒素的多种生物学效应，本研究通过体外实验探究了人结肠上皮细胞的基因转录变化情况。
实验设计概述：将HCT-8细胞以100 ng/ml的浓度分别用毒素A（TcdA）或毒素B（TcdB）处理；分别于添加毒素后的2小时、6小时及24小时，从未经处理的对照组及毒素处理的细胞中分离RNA。