Genetic variation in feral hemp populations across the Nebraska climate gradient

The objective in this study was to develop and characterize the chemistry and genetics of a collection of feral hemp germplasm from across Nebraska for use in a hemp breeding program. An additional goal was to compare the chemistry and genetics of male and female flower structures. RNA was extracted from isolated, dissected flower tissue of one female and one male plant from each of three populations (total six samples). These plants were derived from seeds collected in Knox, Madison, and Merrick counties. Illumina reads were obtained from the RNA extracts, mapped onto a C. sativa reference genome, and gene expression levels were determined. mRNAs in female and male flower tissues

本研究旨在开发并表征采自美国内布拉斯加州各地的野生大麻种质资源集合的化学与遗传特性，以供大麻育种项目使用。本研究的另一目标为比较雌雄花结构的化学组成与遗传特征。研究人员从三个种群（种子分别采自诺克斯县、麦迪逊县与梅里克县）的各一株雌株与雄株的分离解剖花组织中提取RNA，总计获得6份样本。对上述RNA提取物进行Illumina测序，将得到的读段比对至大麻（Cannabis sativa）参考基因组，并测定基因表达水平。雌、雄花组织中的mRNA