Finding genomic targets of transcription factor Six3 in neural stem cells.

Pro-neural transcription factor Six3 has been implicated in neuronal differentiation. We established primary neural stem cell with Six3 overexpression, and utilized CUT&RUN to profile genomic loci bound by Six3. We cultured primary neural stem cells from mice and overexpressed Six3 in the cell lines. We differentiated the stem cells into neural lineage and performed CUT&RUN to profile genomic regions bound by Six3.

促神经转录因子（Pro-neural transcription factor）Six3 已被证实参与神经元分化过程。我们构建了过表达Six3的原代神经干细胞（primary neural stem cell），并利用CUT&RUN技术绘制Six3的基因组结合位点图谱。我们从小鼠体内分离培养原代神经干细胞，并在该细胞系中过表达Six3。我们将这些干细胞诱导分化为神经谱系细胞，并通过CUT&RUN技术分析Six3结合的基因组区域。