Data collection of the PHYCOB cruise with focus on harmfula algal species in the Southwestern Black Sea in 2021@en

The data set was acquired during the PHYCOB cruise in the frame of the Eurofleets+ programme (https://www.eurofleets.eu/2021/10/22/phycob-an-international-oceanographic-expedition-into-the-western-black-sea-coordinated-by-the-alfred-wegner-institut-helmholtz-zentrum-fur-polar-und-meeresforschung-awi/) within the Regional call in the territorrial waters of Romania and Bulgaria in the Western Baltic Sea from 11th to 17th September 2021. The aim of the cruise was to assess the occurrence of potentially harmfal algae and their associated phycotoxins in the Black Sea and the accompanying environmental parameters. Sampling was performed by CTD casts and Rosette water sampling in addition to vertical phytoplankton net hauls from 30 m depth to surface. The determined parameters include the following: Quantitative phytoplankton cell counts in water samples, qulitative determination of toxigenic species next generation sequencing data in plankton net concentrates, monoclonanal cultures established from water sample isolates, phycotoxins in plankton net concentrates, inorganic nitruinets (nitrate/nitrite, phosphate, silicate) , vitamin B12, particulate organic carbon, particulate organic nitrogen, flowcytometry data, and CTD data (temperature, salinity, chlorophyll-a, oxygen, turbidity, radiance).

本数据集采集自PHYCOB航次，该航次隶属于Eurofleets+计划（链接：https://www.eurofleets.eu/2021/10/22/phycob-an-international-oceanographic-expedition-into-the-western-black-sea-coordinated-by-the-alfred-wegner-institut-helmholtz-zentrum-fur-polar-und-meeresforschung-awi/），于2021年9月11日至17日在西波罗的海罗马尼亚与保加利亚领海的区域专项征集项目框架下实施。本航次的研究目标为评估黑海海域潜在有害藻类及其相关藻毒素的检出情况，以及配套的环境参数。采样工作采用CTD（温盐深仪，Conductivity-Temperature-Depth）投放与罗塞塔采水器取水相结合的方式，同时辅以从30米水深至表层的垂直浮游植物网拖采样。本次测定的参数涵盖以下类别：水样中的浮游植物细胞定量计数、浮游植物网滤浓缩物中产毒物种的定性检测与下一代测序（Next Generation Sequencing）数据、从水样分离物中构建的单克隆培养物、浮游植物网滤浓缩物中的藻毒素、无机营养盐（硝酸盐/亚硝酸盐、磷酸盐、硅酸盐）、维生素B12、颗粒有机碳、颗粒有机氮、流式细胞术（Flow Cytometry）数据，以及CTD采集的温度、盐度、叶绿素a、溶解氧、浊度、辐亮度等参数。