iPSC nanovesicles cardiac cell repair

Here, we engineered functional EV-like nanovesicles (NVs) from human-induced pluripotent stem cells and highlight their potential as a functional surrogate for natural EVs for tissue repair. Proteome profiling of target cells further identified pathways implicated in angiogenesis (MYH10, AAMP, SLC3A2), cell survival (CCN2, RHEB, IGF2R, MFGE8), and fibroblast activation (CCN2, STAT3, COL1A1/2/4A2/12A1, ACTN1/4, ACTC1). This study demonstrates scalable generation of functional NVs and highlights their potential as a functional surrogate for natural EVs for tissue repair.

本研究从人诱导多能干细胞中工程化构建了功能活性的细胞外囊泡样纳米囊泡（EV-like nanovesicles，NVs），并阐明其作为天然细胞外囊泡功能替代物用于组织修复的应用潜力。通过对靶细胞进行蛋白质组谱分析，本研究进一步鉴定出与血管生成（MYH10、AAMP、SLC3A2）、细胞存活（CCN2、RHEB、IGF2R、MFGE8）及成纤维细胞活化（CCN2、STAT3、COL1A1/2/4A2/12A1、ACTN1/4、ACTC1）相关的信号通路。本研究证实了功能性NVs的规模化制备可行性，并再次凸显其作为天然细胞外囊泡功能替代物用于组织修复的潜在价值。