Supporting Information S1 - Emergent Functional Properties of Neuronal Networks with Controlled Topology

Analysis of the average neurite extension of mCherry-transfected neurons grown in grid patterns. The average neurite length was evaluated by transfecting a limited number of neurons in the network with mCherry. The transfection protocol demonstrates that the imposition of the pattern did not prevent neurites to develop normally as in standard random cultures. Fig. S1 (left panel) shows that the transfection protocol allowed to effectively track neurites of a single neuron up to 800 µm from the soma. The quantification yielded to an average neurite length of 625±81 µm (n = 4). (DOCX)

对网格培养模式下mCherry转染神经元的平均神经突起延伸情况开展分析。本研究通过对培养网络中的少量神经元进行mCherry转染，实现对平均神经突起长度的评估。该转染方案证实，网格培养模式并未阻碍神经突起如同标准随机培养体系中那般正常生长。补充图S1（左面板）显示，该转染方案可有效追踪距离神经元胞体最远达800 μm的单个神经元的神经突起。定量分析结果显示，平均神经突起长度为625±81 μm（n=4）。(DOCX)