DNAse-Seq of Cultured Mouse Podocytes. Mus musculus

Investigation of DNAse Hypersensitivity changes in podocytes cultured under normal or high glucose conditions transfected with a miR-93 mimic or a nontargeting mimic. Overall design: Examination of the changes in hypersensitivity induced by high glucose culture conditions compared to normal glucose conditions to mimic the diabetic millieu. Further, to see if miR-93 overexpression can reverse these changes.

本数据集旨在探究经miR-93模拟物（miR-93 mimic）或非靶向模拟物转染，并分别在正常糖浓度或高糖浓度培养基中培养的足细胞（podocytes）内的脱氧核糖核酸酶超敏位点（DNase Hypersensitivity）变化。整体实验设计：对比模拟糖尿病微环境的高糖培养条件与正常糖培养条件所诱导的超敏位点变化，并进一步验证miR-93过表达能否逆转此类变化。