Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis

Purpose: to determine cellular heterogeneity of the murine colonic epithelium and mesenchyme; Summary: We determined 16 disctinct sub-populations of colonic epithelial and mesenchymal cells. Further we found 3 distinct sub-populations of colonic PdgfraEGFP-positive fibroblasts. Wildtype murine colon cells were sorted using FACS (using EpCAM and CD45 antibodies). PdgfraEGFP intestinal mesenchymal cells were sorted using FACS (GFP positvity). Single cell RNA sequencing was perfomed (for details see Brügger et al, in prep)

研究目的：解析小鼠结肠上皮与间质组织的细胞异质性；研究概况：本研究共鉴定得到16种结肠上皮细胞与间质细胞的独特亚群，此外还发现3种结肠PdgfraEGFP阳性成纤维细胞亚群。实验过程中，研究人员通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）结合EpCAM与CD45抗体分选野生型小鼠结肠细胞，并借助FACS基于GFP阳性特征分选PdgfraEGFP标记的肠道间质细胞，随后对分选获得的细胞开展单细胞RNA测序，详细实验细节参见Brügger等人的待发表研究。