Localization of SIV infected cells in LN from SM and PM.

Infected cells, as quantified by ISH for SIV RNA. Histomorphological localization of infected cells (identified by dark blue NBT/BCIP chromogen) in GC versus paracortical microenvironments was accomplished by microscopic analysis of Nuclear Fast Red counterstain. Scoring is based on the average for each group at each time point, divided into quartiles (+; 0–5/mm2) (++; 6–15/mm2); (+++; 16–50/mm2); (++++; >50/mm2) derived from quantitative measurements. Asterisk (*) Indicates one germinal center contained an SIV-infected cell at 2, 6, and 24 wpi in a CD8 depleted SM. Numbers of GC evaluated at each time-point is indicated by n values.

本数据集涵盖通过针对猴免疫缺陷病毒（SIV）RNA的原位杂交（In Situ Hybridization, ISH）定量的感染细胞相关数据。针对感染细胞（以深蓝色NBT/BCIP显色剂标记）在生发中心（Germinal Center, GC）与副皮质微环境中的组织形态学定位，通过对核固红复染样本的显微镜分析完成。评分基于各实验组在各时间点的平均测量值，按四分位区间划分为：(+; 0–5/mm²)、(++; 6–15/mm²)、(+++; 16–50/mm²)、(++++; >50/mm²)，上述分级均源自定量检测结果。星号（*）表示在感染后2、6及24周（wpi）的CD8耗竭SM样本中，单个生发中心存在1个SIV感染细胞。每个时间点所评估的GC数量以n值标注。