Development of microRNA expression signatures for Buyang Huanwu decoction in treating intracerebral hemorrhage

To identify the potential miRNA asscociated with Buyang Huanwu decoction (BYHWD) in treating intracerebral hemorrhage (ICH), we have employed miRNA expression profiling. 126 and 38 miRNAs were identified in the ICH vs sham group and the BYHWD vs ICH group, respectively. Specifically, 15 DEmiRNAs were intersected among the three groups. Expression of 14 miRNA (miR-7b-5p, miR-770-3p,miR-760-3p, miR-376c-5p, miR-1224-5p, miR-298-5p, miR-541-3p, miR-344d-3-5p, miR-653-5p, miR-7a-5p, miR-6540-5p,miR-146a-5p, miR-375-3p, and miR-3962) from this signature was quantified by RT-qPCR. Overall design: Mice were randomly divided into 3 groups (n=5): the Sham, ICH, and BYHWD-H groups. The gavage administration started on the 1st day after the surgery for 14 consecutive days. Brain samples were dectected at 14 d after ICH.

为筛选与补阳还五汤（Buyang Huanwu decoction, BYHWD）治疗脑出血（intracerebral hemorrhage, ICH）相关的潜在微小RNA（microRNA, miRNA），本研究开展了miRNA表达谱分析。在ICH组与假手术组、BYHWD干预组与ICH组中，分别鉴定出126个和38个miRNA。具体而言，三组间共筛选得到15个共有差异表达微小RNA（differentially expressed miRNAs, DEmiRNAs）。本研究通过实时定量聚合酶链反应（Real-time quantitative polymerase chain reaction, RT-qPCR）对该miRNA特征谱中的14个miRNA进行了定量检测，具体包括：miR-7b-5p、miR-770-3p、miR-760-3p、miR-376c-5p、miR-1224-5p、miR-298-5p、miR-541-3p、miR-344d-3-5p、miR-653-5p、miR-7a-5p、miR-6540-5p、miR-146a-5p、miR-375-3p及miR-3962。
实验设计：将小鼠随机分为3组（每组n=5）：假手术组、ICH模型组与补阳还五汤高剂量组（BYHWD-H组）。于造模术后第1日开始灌胃给药，连续给药14天。于ICH造模后14天采集脑组织样本进行检测。