The Epigenetic Regulator UTX Imposes An Effector Differentiation Trajectory in Autoimmune CD8+ T Cell Stem-like Progenitors

Recent findings suggest that long-lived CD8+ progenitors can continuously replenish CD8+ mediators in pancreatic lymph node (pLN). The mechanism controlling this conversion is still unclear. T cell specific UTX-deficiency NOD mice harbor increased frequency of islet-specific progenitors population and decreased frequency of islet-specific mediators. Thus, we analyzed pLN CD8+ T cells of UTXTCD mice and WT mice in 8- and 13-week old NOD mice by scRNAseq. Overall design: We performed scRNAseq on T cells isolated from pancreatic islets of 8-week-old and 13-week-old NOD WT vs UTXTCD females to compare their transcriptome profile.

最新研究表明，长寿型CD8阳性祖细胞（long-lived CD8+ progenitors）可在胰腺淋巴结（pancreatic lymph node, pLN）中持续补充CD8+介导细胞。
目前，调控这一转化过程的分子机制仍不明确。
T细胞特异性UTX缺陷型非肥胖糖尿病（NOD）小鼠的胰岛特异性祖细胞群体频率升高，而胰岛特异性介导细胞的频率则出现下降。
因此，本研究通过单细胞RNA测序（scRNAseq）分析了8周龄和13周龄非肥胖糖尿病（NOD）小鼠中UTXTCD小鼠与野生型（WT）小鼠的胰腺淋巴结CD8阳性T细胞。
整体实验设计：本研究对8周龄及13周龄的雌性非肥胖糖尿病（NOD）小鼠的野生型与UTXTCD组的胰岛分离T细胞进行了单细胞RNA测序，以比较二者的转录组特征。