Base-resolution DNA hydroxymethylation maps of purple sea urchin (Strongylocentrotus purpuratus), european lancelet (Branchiostoma lanceolatum) and zebrafish (Danio rerio) using ACE-seq

Using ACE-seq we investigated single-base resolution hydroxymethylomes of sea urchin, lancelet and zebrafish during development. ACE-seq was perfomred on (i) 24 hpf, 48 hpf, 72 hpf embryos and adult tissue (tube feet) of purple sea urchin in biological replicates; (ii) 36 hpf, larvae (60-70 hpf), juvenile (3-4mo) and adult tissue (liver) of european lancelet in biological replicates (larvae and juvenile stages); (iii) zebrafish 24 hpf embryo.

本研究采用ACE-seq技术，对发育进程中的海胆、文昌鱼与斑马鱼的单碱基分辨率羟甲基化组（hydroxymethylomes）开展检测分析。具体样本与实验设置如下：
(i) 紫色海胆：24 hpf（受精后小时，hours post fertilization）、48 hpf、72 hpf胚胎，以及成体管足组织，所有样本均设置生物学重复；
(ii) 欧洲文昌鱼：36 hpf胚胎、幼虫期（60-70 hpf）、幼体期（3-4月龄）以及成体肝脏组织，其中幼虫与幼体阶段设置生物学重复；
(iii) 斑马鱼：24 hpf胚胎。