RNA-seq of dendritic cell subsets. RNA-seq of dendritic cell subsets

Comparison of cDC1, cDC2, mcDC transcriptome Conventional dendritic cells (cDCs) are classically subdivided in two subsets, named cDC1 and cDC2. We demonstrated that mcDC are a new subset of cDCs. To understand their transcriptomic relatedness, we perform a RNA sequencing on cDC1, cDC2 and mcDCs sorted from mouse spleens. We demonstrate that cDC1, cDC2 and mcDCs all cluster independently in a PCA analysis and an unbiased hierarchical cluster analysis reveals unique gene profile for all three cDC subsets. As mcDC cluster slightly more towards cDC1 than cDC2, we next aimed to more closely examine the transcriptomic relatedness between mcDC and cDC2. We compared the DC subsets gene expression signatures based on a selected gene set known to define cDC2. Both the PCA and hierarchical cluster analysis show that mcDC exhibit a different signature to cDC2. Altogether, we demonstrate that mcDC are a distinct subsets of cDCs with a specific transcriptomic signature. Overall design: splenic sorted cDC1, cDC2 and mcDC

cDC1、cDC2与mcDC转录组比较研究
常规树突状细胞（conventional dendritic cells，cDCs）经典可分为cDC1与cDC2两个亚群。本研究证实，mcDC为一类全新的cDC亚群。为明确三者的转录组相关性，我们对从小鼠脾脏中分选得到的cDC1、cDC2及mcDC进行了RNA测序。
研究显示，在主成分分析（Principal Component Analysis，PCA）与无偏分层聚类分析中，cDC1、cDC2及mcDC均各自独立成簇；且三种cDC亚群均具有独特的基因表达谱。鉴于mcDC的聚类结果更趋近于cDC1而非cDC2，本研究后续进一步深入探究了mcDC与cDC2之间的转录组相关性。我们基于一套已被证实可用于定义cDC2的特征基因集，对各DC亚群的基因表达特征进行了比较。主成分分析与分层聚类分析结果均显示，mcDC与cDC2具有不同的基因表达特征。
综上，本研究证实mcDC是一类具有独特转录组特征的独立cDC亚群。
实验整体设计：从小鼠脾脏中分选得到的cDC1、cDC2及mcDC