Spatial Visium FFPE Samples. Spatial Visium FFPE Samples

Human Formalin fixed paraffin embedded Samples collected from Donated kidney samples, processed for cortex specific sections Overall design: Kidney samples were cut into 6-20 mm sections, fixed in formalin for 16-24hrs, embedded in paraffin, and serial sections cut at 5um. Sections were manually placed on 10x Visum v.10 slides and processed per 10x Visium FFPE Protocols to bind and amplify transcriptome probe panels and generate libraries from mRNA. Data from Healthy cortex was compared to Chronic Kidney disease (CKD) or Acute Kidney disease (AKI)

本数据集的样本取自捐献肾脏组织，为经福尔马林固定石蜡包埋（FFPE）处理、针对肾皮质区域制备的样本。实验设计概述：将肾脏样本切割为6-20毫米的切片，经福尔马林固定16至24小时后进行石蜡包埋，随后切成厚度为5微米的连续切片。将切片手动放置于10x Visium v10 玻片上，依照10x Visium FFPE实验流程进行处理，完成转录组探针组的杂交与扩增，并从mRNA中构建测序文库。本研究将健康肾皮质的测序数据与慢性肾脏病（CKD）或急性肾脏病（AKI）患者的样本数据开展对比分析。