Microbial communities in uranium mine tailings: high-throughput sequencing data

In this study we characterized microbial communities in tailings from a uranium mine waste facility, the Rabbit Lake In-Pit Tailings Management Facility (RLITMF), in Northern Saskatchewan, Canada. We sampled two RLITMF boreholes in summer 2014, taking samples from a number of depths in both boreholes. We used high-throughput amplicon sequencing (Illumina MiSeq platform) of the 16S rRNA gene (V4 region) to look at bacterial and archaeal diversity in the samples.

本研究针对加拿大萨斯喀彻温省北部的雷比特湖坑内尾矿管理设施（Rabbit Lake In-Pit Tailings Management Facility，RLITMF）的尾矿样品，解析其中的微生物群落特征。本研究于2014年夏季对该设施的2个钻孔开展采样工作，分别从两个钻孔的多个深度获取了样品。我们采用依托Illumina MiSeq测序平台的16S核糖体RNA基因（V4区）高通量扩增子测序技术，对样品中的细菌与古菌多样性进行分析。