The brlA gene is involved in conidiogenesis and secondary metabolism in Penicillium expansum. The brlA gene is involved in conidiogenesis and secondary metabolism in Penicillium expansum

In Aspergillus sp., BrlA is a master transcription factor essential for the induction of conidiogenesis. Sometimes it is also involved in the secondary metabolism. The aim of this study was to characterize the role of BrlA in Penicillium expansum. For that a null mutant PeΔBrlA was generated. Overall design: PeΔBrlA and WT strains were grown in the same conditions ( 25°C for 5 days in the dark) on MEA medium. The RNAs were extracted and their transcriptomes were analyzed.

在曲霉属（Aspergillus sp.）真菌中，BrlA是介导产孢过程必不可少的核心转录因子，同时也可参与次级代谢调控。本研究旨在解析BrlA在扩展青霉（Penicillium expansum）中的功能，为此构建了BrlA基因敲除突变体PeΔBrlA。
整体实验设计：将PeΔBrlA突变体与野生型（WT）菌株接种于麦芽提取物琼脂（MEA）培养基上，在相同条件（25℃暗培养5天）下培养；随后提取总RNA并对两组样本的转录组进行分析。