Microarray gene expression profiling of isolated class III Drosophila dendritic arborization sensory neurons

Class III Drosophila dendritic arborization sensory neurons were isolated via magnetic bead sorting and total RNA isolated from the samples was used for gene expression profiling to explore molecular functions of this sensory neuron subtype. Gene expression profiling of class III da neurons, isolated via magnetic bead sorting (using protocols as described in Iyer et al., 2009), was performed at the third instar larval stage of development. 40-50 aged-matched third instar larvae of genotype ppk-Gal4,UAS-mCD8::GFP,ppk-Gal80 (labels class III dendritic arborization sensory neurons (Iyer et al., 2013)) were used to perform independent class III-specific cell isolations. These datasets were also directly compared with GSE46154 containing microarray datasets for class I and class IV dendritic arborization sensory neurons and age matched whole larval homogenate RNA.

本研究通过磁珠分选（magnetic bead sorting）分离得到果蝇（Drosophila）第三类树突分支感觉神经元（dendritic arborization sensory neurons），提取样本中的总RNA用于基因表达谱分析（gene expression profiling），以探究该感觉神经元亚型的分子功能。本研究在发育的三龄幼虫阶段，对通过磁珠分选（遵循Iyer等人2009年发表的实验方案）获得的第三类da神经元开展基因表达谱分析。实验采用40~50只基因型为ppk-Gal4,UAS-mCD8::GFP,ppk-Gal80的年龄匹配三龄幼虫，该基因型可标记第三类树突分支感觉神经元（Iyer et al., 2013），用于独立的第三类特异性细胞分离实验。本数据集还与GSE46154数据集进行了直接比对，后者包含第一类、第四类树突分支感觉神经元的微阵列（microarray）数据集以及年龄匹配的全幼虫匀浆总RNA。